1 Given the following information for a rate reaction, calculate the activity of
ID: 87517 • Letter: 1
Question
1
Given the following information for a rate reaction, calculate the activity of a serum specimen for alanine aminotransferase in international units per liter (IU/L). (2 pts)
Time
Absorbance
1 min
1.104
Specimen volume= 20 uL
2 min
1.025
Reagent volume= 3.0 mL
3 min
0.950
Molar absorptivity for NADH at 340 nm= 6.22 x 103 L/mol cm
4 min
0.873
Light path= 1 cm
186
198
1857
1869
2
A 42 yr old male presents with anorexia, nausea, fever and icterus of the skin and mucous membranes. He noticed that his urine had appeared dark for past several days. The following biochemical tests were resulted. What is your opinion of the most likely diagnosis?
ALP slightly elevated
ALT markedly elevated
AST markedly elevated
GGT slightly elevated
TBIL moderately elevated
Urine bilirubin positive
Fecal urobilinogen decrease
3
Given the serum protein electrophoresis pattern shown, which transaminase (AST –ALT) results would you expect
Time
Absorbance
1 min
1.104
Specimen volume= 20 uL
2 min
1.025
Reagent volume= 3.0 mL
3 min
0.950
Molar absorptivity for NADH at 340 nm= 6.22 x 103 L/mol cm
4 min
0.873
Light path= 1 cm
Explanation / Answer
Answer:
1) Based on the information given:
2) A 42 yr old male presents with anorexia, nausea, fever and icterus of the skin and mucous membranes. He noticed that his urine had appeared dark for past several days. The following biochemical tests were resulted. What is your opinion of the most likely diagnosis?
Minute Absorbance 1 1.104 2 1.025 3 0.95 4 0.873Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.