solvent front Component A Component 3 Component C Spotting line You spot an unkn

Question

solvent front Component A Component 3 Component C Spotting line You spot an unknown analgesic drug on a TLC plate, develop the plate, then visualize it. The resulting TLC plate is shown above. The overall length of the plate is 10.0 cm. Your spotting line is 1.5 cm from the bottom of the plate. You mark the solvent front 1.0 cm from the top of the plate. You measure the three spots and find that Component A traveled 6.3 cm, Component B traveled 4.0 cm, and Component C traveled 2.1 cm. Calculate the Rf value for each of the three components. 2. What are the two methods of visualization used in this TLC experiment? UV y You have just run a TLC of a three component mixture, using a silica gel plate and an 80:20 mixture of chloroform and methanol (mobile phase). Your goal is to purify spot B(0.52) away from spots A (0.23) and C (0.81), with their Rfs given in parentheses. However, to purify a compound on TLC, you've been told that having an Rf of 0.3 of the spot to be purified is ideal. Which solvent would you add more of in the mobile phase to achieve this?

Explanation / Answer

1) Rf value= Distance travelled by given component / Distance travelled by solvent

Hence, for given Components A, B and C

For A , 0.23

For B, 0.52

For C, 0.81

2) Two visualisation methods for TLC are as follows:

i) Under UV Lamp

ii) Iodine Chamber

3) We will add more methnol in the mobile phase because of its higher polarity.

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