64) Secondary structure of protein is maintained by .. 65) Non-covalent bonds in

Question

64) Secondary structure of protein is maintained by

..

65) Non-covalent bonds involved in forming correct structures of proteins are labile to heat. What does it mean for you to handle samples containing proteins?

..

66) Both hexokinase and glucokinase phosphorylate glucose, but have different affinity. Km for glucokinase is 10mM and Km for hexokinase is < 1mM. The blood glucose level for normal range is 77 to 99mg/dL (4.28mM to 5.5mM). When would both enzyme function?

..

67) Maine vitamin B2 family is FAD (flavin adenine dinucleotide) and FMN (flavin mononucleotide). FMN is used as a coenzyme in many reactions and FAD is used for transfer of energy such as forming FADH2 during TCA cycle and oxidative phosphorylation. Some amino acids are involved in the processes of methylation of DNA and histone proteins. What will be a result of restricting food intake?

,,

68) What is the role of uncoupling protein in mitochondrial membrane of brown adipose tissues?

Explanation / Answer

#64 secondary structure of proteins such alpha-helix and Beta-sheets are formed via hydrogen bonds between the H of amine group and the O of the carbonyl group. To maintain the structure, these hydrogen bonds must not be broken.

#65 Since protein structures will change (denature) when exposed to heat, protein samples must be handled with least exposure to heat. This would require keeping a protein sample in cold temperatures.

#68 The uncoupling protein (UCP1) in mitochondrial membrane in brown adipose tissues acts as proton transporter which dissipates the proton gradient created by the respiration as heat. This results to uncoupling of oxidative phosporyllation.

Related Questions

Navigate

• Browse (All)
• Browse 6
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.