Do Calum F and H show work Whem the DF is not equal to 50 , instead its equal to

Question

Do Calum F and H show work Whem the DF is not equal to 50 , instead its equal to 200 !!

DF= 200

nome work Table 1 a. Total Cells Total Cells Side 1 of Side 2 of Total Ave DF #viable Volume Total of Hemocytometer Hemocytometer Viable Viability Cells/ml in the Cells in Cells ilopaque blue wopaque T blue cells cells cells cells Flask the Flask Saccharomyces cerevisiae Stock 50 200m 1. Complete Table 1 from the cells counts obtained in Procedure 1. Calculate the cell concentration, viability, and the total number of cells in the f Please show your work. (3pt) of cells) x loo 25.53 X 00 (605)

Explanation / Answer

Dead cells take up tryphan blue stain and appear blue

Total number of cells (dead and live) = 128 +343+80+262 = 813

Average of Viable cells= (128+80)/2 = 104

% viability = Viable cells/total cells x 100 = (128+80)/813 x 100 = 25.5%

Dilution factor = 50

Viable cells/mL = Avg live cells x DF x 10^4

Viable cells/mL = 104 x 50 x 10^4 = 52 x 10^6 cells/mL

Total volume in flask= 200mL

Total number of cells in flask = 52 x 10^6 cells x 200 = 10.400 x 10^9 cells

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