A student placed 50 mL of a mobile phase in a standard 250 mL beaker, added a pi
ID: 867678 • Letter: A
Question
A student placed 50 mL of a mobile phase in a standard 250 mL beaker, added a pice of filter paper and sealed the top with aluminum foil. while waiting for the air above the mobile phase to become saturated with vapors, the student prepared a TLC plate by drawing a pencil line across the plate 3 mm from the bottom edge. The student then placed several sample spots along the pencil line and used a flourescent light to verify that the spots were of an appropriate size. The student then placed the plate in the chamber and allowed it to develop until the solvent front had traveled approximately 10 cm. When the student attempted to analyyze the plate, the spots were not visible anywhere on the plate. What is the most likely reason for this observation.
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