laboratory Review 2 1. Make the following conversions: liter c, 50 ml cm b, 15 m

Question

laboratory Review 2 1. Make the following conversions: liter c, 50 ml cm b, 15 mm d. 5 g 2. Explain the designation "compound light" microscope: a. compound b, light 3. What function is performed by the diaphragm of a microscope? 4. Why is it helpful for a microscope to be parfocal? 5. Why is locating an object more difficult if you start with the high-power objective than with the low-power objective? 6. How much larger than actual size does an object appear with a low power objective? 7. A virus is 50 nm in size. a. Would you recommend using a stereomicroscope, a und light microscope, or an electron microscop Why? to see it? b. How many micrometers is the virus? um 8. If the diameter of a field is 1.6 mm, and you count 40 consecutive cells from one end of the field to the other, h wide is each cell in micrometers? 9. What type of microscope, aside from the compound light microscope, might you use to observe the organisms found in pond water? 10. Briefly describe the necessary steps for observing a slide at low power under the compound light microscope. McGraw-Hill Access Science Website LEARNSMAR An online encyclopedia of science and technology

Explanation / Answer

Q.NO. 1

(a) 1mm=1000m = 0.1 cm

(b) 15mm= 1.5 cm = 15000 m

(c) 50 ml = 0.05 L

(d) 5gm = 5000mg

Q.NO 2

(a) a compond is called compond because it consists of two lense i.e eye piece or ocular lense and objective lense unlike simple microscope which consists of only single lense ,the primary image obtained by objective lense in compound microscpe is compounded/multiplied by eye lense/ocular lense both the lenses are fixed in the same tube

other componets of compound microscope are

1..condenser :- to adjust the intensity of light

2 Coarse adjustment knob/screw:- for rapid focusing

3 Fine adjustmet knob/screw :- for sharp and fine focusing

4.illuminator ;- to iluminate the object

5 Stage :- where objectto be observed is placed

Diaphragm:- under the stage to vary the intensity of light

, objective lense has short focal length while eye piece has large focal length , objective lense is is near the object to be observed.compond microscope can magnify the image 1000 to 2000 times the original size of object

(b) Light ;- since compound light microscope uses light as source of illumination unlike Electron microscope which uses electron beam hence it is called 'compound light microscope'

Q.No.3

Diaphragm is a rotating disc like componet of compound microscope it consits of holes which is used to control the intensity and size of the cone which is projected upward on the object to illuminate it according to the requirement ,

Q.no 4

parafocal microscope has a property in the microscope which allows to adjust each objective in a way that it remains in relative focus with the other objectives on the microscope when we change or switch from one magnification to another ,parafocalling prevents eye strain and wear and tear that can occur parafocalling is not present.

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