2.5 Microscopic Observations Figure 2.10 Preparation When a specimen is prepared
ID: 86727 • Letter: 2
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2.5 Microscopic Observations Figure 2.10 Preparation When a specimen is prepared of a wet mount. for observation, the object should always be viewed as a wet mount. A wet mount is prepared by placing a drop of liquid on a slide or, if the material is dry, by placing it directly on the slide and adding a drop of water or stain. The mount is then covered with a coverslip, as illustrated in Figure 2.10. Dry the bottom of your slide before placing it on the stage. b. Lower ooverslip slowly a. Add drop of liquid or dry object and liquid. Methylene blue Awold contact with skin, eyes and mucous membranes. Exercise care in using this chemical, any should spil on your skin, wash the area with mild soap and water. Methylene blue wil also stain clothing. Follow your instructor's drectors for its disposal. Observation Onion Epidermal Cells Epidermal cells cover the surfaces of plant organs, such as leaves. The bulb of an Scalpel Exercise care onion is made up of fleshy leaves. when using a scalpel. 1. With a scalpel, strip a small, thin, transparent layer of cells from the inside of a fresh onion leaf. 2. Place it gently on a clean, dry slide, and add a drop of iodine solution (or methylene blue). Cover with a coverslip. Figure 2.11 Onion epidermal cells. 3. Observe under the microscope. 4. Locate the cell wall and the nucleus. Label Label the cell wall and the nucleus. Figure 2.11 5. Count the number of onion cells that line up end to end in a single line across the diameter of the high- power (40X) field. Based on what you learned in Section 24 about measuring diameter off field, what is your high- Pm Calculate the length of each onion cell (HPD number of cells): Am Wash and reuse this slide for the next exercise (Human Epithelial Cells). 24 Laboratory 2 Metric Measurement and MicroscopyExplanation / Answer
4 ans-1-Nucleus and 2- cell wall
5 ans-The number of onion cell that line up end to end in a single line -approximate 4
low power objective = 10x
High power object -40x
Diameter will be 10x *40x = 400x
length of the each onion cell-HPD /4 = 400/4 = 100mm
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