Fill out table and show all calculations Final Volume of Volume of Volume of Fin

Question

Fill out table and show all calculations

Final             Volume of              Volume of               Volume of       Final volume (uL)

[PNPP] mM   0.5mM PNPP(ul)   0.2M Tris-HCl(ul)   enzyme (uL)

0.01                                                                            100                        1500

0.02                                                                             100                       1500

0.04                                                                             100                         1500

0.06                                                                            100                            1500

0.08                                                                            100                              1500

0.1                                                                           100                             1500

0.2                                                                              100                               1500

0.3                                                                           100                               1500

0.4                                                                             100                                1500

Explanation / Answer

1. total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.01 X 1500 / 0.5 = 30microL

so volume of 0.2 M Tris HCl = 1500-(30+100) = 1370 micro L

2. total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.02 X 1500 / 0.5 = 60microL

so volume of 0.2 M Tris HCl = 1500-(60+100) = 1340 micro L

3.total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.04 X 1500 / 0.5 = 120microL

so volume of 0.2 M Tris HCl = 1500-(120+100) = 1280 micro L

4. total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.06 X 1500 / 0.5 = 180microL

so volume of 0.2 M Tris HCl = 1500-(180+100) = 1220 micro L

5. total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.08 X 1500 / 0.5 = 240microL

so volume of 0.2 M Tris HCl = 1500-(240+100) = 1160 micro L

6.total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.1 X 1500 / 0.5 = 300microL

so volume of 0.2 M Tris HCl = 1500-(300+100) = 1100 micro L

7. total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.2 X 1500 / 0.5 = 600microL

so volume of 0.2 M Tris HCl = 1500-(600+100) = 800 micro L

8. total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.3 X 1500 / 0.5 = 900microL

so volume of 0.2 M Tris HCl = 1500-(900+100) = 500 micro L

9. total volume of solution = 1500micro L

Volume of enzyme = 100 micro L

Volume of 0.5mM = 0.4 X 1500 / 0.5 = 1200microL

so volume of 0.2 M Tris HCl = 1500-(1200+100) = 200 micro L

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