Autologous smooth muscle cells (SMCs) were transfected with plasmid constructs c

Question

Autologous smooth muscle cells (SMCs) were transfected with plasmid constructs containing the full coding sequence for endothelial nitric oxide synthase using liposome mediated gene transfer. The genetically modified SMCs produced high levels of nitric oxide, an agent typically produced by endothelial cells to prevent platelet adhesion. In an effort to improve the blood compatibility of an expanded PTFE vascular prosthesis surface-modified with RGD peptides. genetically modified autologous SMCs were seeded on the prosthesis prior to implantation. Although platelet adhesion was abrogated and patency restored in the short-term, use of the SMC/polymeric prosthesis was soon discontinued due to implant failure. What was the likely cause and mechanism of implant failure?

Explanation / Answer

Autologous smooth muscle cells That have been transfected with plasmid containing nitric acid synthase and is capable of producing high level of nitric oxide, thus platelet adhesion was stopped. Thus, first part was successful in which the smooth muscle cells would mimic the action of endothelial cells and no platelet adhesion. Due to this property of no platelet adhesion, the endothelial cells are inefficient in being seeded onto the vascular prostheses. Thus, the failure of the implant .

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