The normal DNA sequence that codes for two adjacent codons along the length of a

Question

The normal DNA sequence that codes for two adjacent codons along the length of an mRNA is as follows: TGAGGA. Based on the wobble theory, which of the following mutations in the DNA sequence would be LEAST likely to cause a problem? TTAGGA AGAGGA TGTGGA TGAAGA One method of DNA sequencing uses dideoxynucleotides. The incorporation of a dideoxynucleotide stops any further DNA synthesis because: the dideoxynucleotide lacks a 3'-hydroxyl group so another nucleotide cannot attach to it the dideoxynucleotide lacks a 5'-phosphate group so another nucleotide cannot attach to it the dideoxynucleotide cannot bind to the RNA primer the dideoxynucleotide cleaves the DNA polymerase necessary for DNA synthesis Where does translation occur for proteins destined to be imbedded in the membrane or excreted from the cell? in the free cytoplasm in the plasma membrane in the rough endoplasmic reticulum in the smooth endoplasmic reticulum Which of the following processes is an exception to the "central dogma" and typically only occurs in viruses? DNA makes RNA RNA makes DNA RNA makes polypeptide Polypeptide makes DNA During excision of a viral genome from the host chromosome, the virus accidentally takes a piece of host DNA with it. This may occur: while the virus is converting from a lytic to lysogenic stage resulting in generalized transduction at anytime while the virus is converting from a lysogenic to lytic stage

Explanation / Answer

1) The answer is c. According to wobble theory, mutation in the third nucleotide rarely affects the protein sequence due to degeneracy of genetic code.

2) The answer is a. Dideoxynucleotide lacks a 3' hydroxyl group so another nucleotide cannot attach to it and the DNA synthesis is terminated.

3) The answer is c. the translation of proteins that are destined to incorporate in the membreane or excreted from the cell are produced by the ribosomes present on the rough endoplasmic reticulum.

4) The answer is b. only viruses have the ability to generate DNA from RNA due to presence of reverse transcriptase enzyme.

5) The answer is d. when the virus converts from lysogenic to lytic stage, a piece of host DNA is taken by the virus during a process known as Specialized transduction.

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