Which of the following levels of protein structure is most important for determi

Question

Which of the following levels of protein structure is most important for determining the overall 3-dimensional configuration of the protein essential for proper protein function: primary structure secondary structure tertiary structure quaternary structure If Southern blot analysis is used to determine if a specific gene is present or not in a patient's sample, which of the following steps is NOT involved in the analysis process: treat DNA sample with restriction enzyme(s) and run on an electrophoresis gel transfer the DNA from the gel to a membrane filter allow DNA probe for the gene hybridize to the DNA on the membrane filter to determine if gene is present isolate RNA from the patient's sample and hybridize it to the DNA on the membrane filter Which of the following statements is true regarding amniocentesis and chorionic villus sampling (CVS) both procedures collect cell samples that can be tested for chromosome analysis and DNA testing amniocentesis is typically performed at 10-12 weeks gestation, while CVS is usually done at 16 weeks amniocentesis involves taking a solid mass of tissue for analysis, CVS requires taking a fluid sample amniocentesis can be used for chromosome analysis but not DNA analysis, and vice versa for CVS The Golden Rice was genetically modified to contain high levels of precursor to vitamin A by: ding rice and carrot plants together and creating new hybrid strains adding random segments of the carrot genome to the rice genome using restriction enzymes adding vitamin A in the soil of the rice plants adding specific genes from daffodils or maize to the rice genome using recombinant DNA technology Prions are: a form of RNA that is involved in splicing out introns a virulent type of bacteria that causes "mad cow disease" in cattle and humans an infectious agent entirely made up of abnormal protein that causes Creutzfeldt Jakob disease and fatal insomnia in a group of diseases that are caused by viruses that are able to jump from one species to another, e.g. cows to humans Genetically engineered vaccines, such as for hepatitis B and human papillomavirus, are a relatively new type of vaccine called subunit vaccines inactivated vaccines attenuated vaccines gardasil vaccines Hypothyroidism is an AUTOSOMAL RECESSIVE condition. If a couple already has a phenotypically normal son and they just found out that their newborn daughter has Hypothyroidism, what is the probability that the older son is a CARRIER for Hypothyroidism? 1/4 1/2 2/3 100%

Explanation / Answer

1) tertiary structure. polypeptide folds into a compact, 3-D shape stabilised by interactions between R groups of amino acids to form active protein.

2) isolate RNA from patients sample and hybridize it to the DNA on the membrane filter.

3) both procedures collect cell samples that can be tested for chromosome analysis and DNA testing.

4) adding specific gene from daffodils or maize to the rice genome using rDNA technology.

5) an infectious agent entirely made up of anormal protein that cause CJ disease and fetal insomnia in human.

6) subunit vaccine

7) there is a 50% (1/2) chances that son have hypothyroidism.

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