Deduce the sequence of amino acids in the peptide using the following obervation

Question

Deduce the sequence of amino acids in the peptide using the following obervations made after a sample of the peptide was subjected to each of the reagents a-e Trp, Tyr, Phe, Met, Gly, Lys, Arg, Arg

a. Edman degradation result: PTH-Gly. The remaining fragment subjected to another round of Edman degradation resulted in PTH-Tyr

b. Chymotrypsin result: fragmentend the peptide into two dipeptides and a tetrapeptide. Carboxypeptidase treatment of the tetrapeptide showed that the amino acid Phe is at the C- Terminal end.

c. Carboxypeptidase A treatment: resulted in releasing amino acid Phe

d. CNBr treatment: fragmented the peptide to give a pentapeptide and a tripeptide. The tripeptide contained Phe

e. Digestion with Trypsin gave the following four fragments: Lys, Phe, Two tripeptides

Explanation / Answer

these three are correct

a. Edman degradation result: PTH-Gly. The remaining fragment subjected to another round of Edman degradation resulted in PTH-Tyr

b. Chymotrypsin result: fragmentend the peptide into two dipeptides and a tetrapeptide.

d. CNBr treatment: fragmented the peptide to give a pentapeptide and a tripeptide. The tripeptide contained Phe

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