b. sole source of lipids d. sole source of proteins 22. In the Durham tube ferme
ID: 86375 • Letter: B
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b. sole source of lipids d. sole source of proteins 22. In the Durham tube fermentation test, each sugar broth is supplemented a. with beef extracts or peptone c. a specific carbohydrate e, a and b b. with citrate as sole source of carbon d. a and c 23. The pH indicator phenol red a. green at acidic value c. yellow at acidic value b, red at acidic value d. black at acidic value 24. If an organism ferment a sugar d. a or a and coccur a. acid is usually produced e, (b) and (c) occur b. base is usually produced c. gas may also be and end product 25. E coli is a, a glucose fermenter c. a rod shaped bacterium e. none of the above b. a gram negative bacterium d all of the above 26. Methyl Red test is an important test in differentiating genera such as a. Escherichia, and Salmonella d, a and b e, a and c b Proteus, and Aeromonas c, mumps virus and Porphyromonas 27. In the O/F glucose test, if an organism can metabolized glucose aerobically and anaerobically, this organism is said to be a. fermentative and oxidative in its metabolism c. oxidative in its metabolism b fermentative in its metabolism d, none of the above 28. Which of the following microorganisms will be positive for the citrate test? a. Enterobacter aerogenes c. Pseudomonas aeruginosa d. none of the above b, E coli 29. Cytochrome oxidase occurs in a. bacteria that carry out fementation c, Pseudomonas e, c band b. bacteria that carry out oxidation d. a and b 30. Hydrogen peroxide is a highly reactive oxidizing agent that can damage essential molecules in the bacterial cell. To avoid this damage, aerobes produce the enzyme d., caseinase a. catalase b, deaminase c. lipase 31. The enzyme nitrate reductase catalyzes a. the transfer of electron from cytochrome b to nitrate, reducing it to nitrite b. the transfer of electron from nitrate to cytochrome b c, the transfer of electron from cytochrome b to nitrite, reducing it to nitrate d, none of the above transfersExplanation / Answer
22. d - In Durhamtube Fermentation test ,Nutrient sugar broth is used.
23. c - pH indicator Phenol red turns Yellow at acidic value.
24. d - If Organism ferment the sugar then acid and gas or only acid produced.
25. d - E.coli is gram negative,rod shaped and glucose fermentative organism.
26. d - Methyl red test is used to differentiate the bacteria of Enterobacteriaceae family.
27. b - if an organism metabolize glucose aerobically and anaerobically then it said to be fermentative organism.
28. c - Pseudomonas aeruginosa give positive citrate test because it has capacity to used citrate as sole source of carbon.
29. e - Cytochrome oxidase present in bacteria who carry out oxidation e.g. Pseudomonas.
30. a - An organism produced catalase to protect from hydrogen peroxide.
31. a - The enzyme nitrate reductase catalyzes transfer of electron from cytochrome b to nitrate riteand reducing it to nitrite.
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