Match the descriptions with the proper technique. Note: Some items are duplicate

Question

Match the descriptions with the proper technique. Note: Some items are duplicated so that they can be used twice. Thin-layer chromatography High-performance liquid chromatography Adsorption chromatography Gas-liquid chromatography primarily used to separate mixtures separates large volumes primarily used to separate mixtures of methylated fatty acids of lipid mixtures of methylated fatty acids (copy) small samples are applied to a column is packed with silica gel narrow bore column is highly pressurized silica gel coated glass plate to force sample through rapidly specific lipids are identified specific lipids are identified by their elution time by their elution time (copy)

Explanation / Answer

1) Thin Layer Chromatography

primarily used to seperate mixtures of methylated fatty acid

small samples are applied to a silica gel coated glass plate

2)High Performance Liquid Chromatography

narrow bore column is highly is pressurised to force sample through rapidly

Specific lipids are identified by their elution time

3)Adsorption Chromatography

Column is packed with silica gel

primarily used to seperate mixtures of methylated fatty acids

4)Gas Liquid Chromatography

Specific lipids are identified by their elution time

Seperate large volumns of lipid mixtures

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