Which of the following techniques would allow you to examine the timing and loca

Question

Which of the following techniques would allow you to examine the timing and location of a gene’s expression as well as the size(s) of the

transcripts produced from the gene?

a) in situ hybridization

b) microarray analysis (WRONG)

c)northern blotting

d) Southern blotting

Which of the following techniques can NOT tell you in what type of cell a gene is expressed?

a) Southern blot

b) in situ hybridization

c) microarray analysis

western blot (WRONG)

What would be the expected effect of a loss­of­function mutation in PRC2?

Increased expression of X­linked genes

Failure to pass on histone methylation markers to subsequent generations

Widespread reductions in gene expression (wrong choice)

A and B

B and C

Explanation / Answer

Answer 1) The correct answer is option (C).

Both Northern blotting and in situ hybridization techniques allow examining the location and timing (when genes are transcribed) and location of the genes. But Northern blotting is the only technique that provides the information regarding transcript size.

Answer 3) The correct options are A and B.
PRC2 is the class of polycomb-group protein which consists of histone methyltransferases. The enzyme histone methyltransferase is responsible for the decreased expression of the gene. It causes the methylation of histone proteins of the chromatin and thereby turns off the transcription of the gene. PRC2 is required for the silencing of genomic region. It plays an important role in X-inactivation (dosage compensation).

Hence, a loss of function mutation in PRC2 increased the expression of X-linked gene and failure to pass on histone methylation markers to subsequent generations.

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