Which antiseptic is the most effective against staph aureus? Which antiseptic is

Question

Which antiseptic is the most effective against staph aureus? Which antiseptic is the most effective against Pseudomonas aeruginosa? What's the purpose of this lab and the observation and conclusion? B 16: EFFECTIVENESS OF ANTISEPTICS Introduction l. "Anti" refers to against, and "septics" (from sepsis) refers to microbial growth 2. Antiseptics are chemical agents used on the human body (i.e. hands) for inhibiting or killing bacteria. 3. The United States Food and Drug Administration (FDA) sets official guidelines in evaluating the effectiveness of antiseptics (and disinfectants, to be used on nonliving objects) 4. The following study using the filter paper disk method is the most common and simplest way to evaluate how well antiseptics work against microbial growth Procedure o mm, 1. This lab is done in a group at each table 2. Each group should obtain 2 nutrient agar plates, 2 sterile cotton swabs, and 6 filter paper disks 3. Using a cotton swab, inoculate one plate with Staph aureus (completely streak the surface of agar) 4. Using another cotton swab, inoculate the second plate with Pseudomonas aeruginosa (completely streak .5 (Al cohol) the surface of agar) 5. Place 2 filter paper disks into phenol, another 2 into formaldehyde, and the last 2 into iodine 6. On each agar plate, place 3 filter paper disks (one of each type of antiseptic) 7. Label the plates with the names of bacteria, and your name 8. Place the plates into the designated racks inverted for incubation 9. In the next lab period, recover your 2 plates 10. Each plate should show cloudy bacterial growth on the agar surface 11. Surrounding each filter paper disks, a clear "zone of inhibition" (where bacterial growth is absent) can now be measured using a ruler 12. the zones of inhibition for all disks in millimeters (mm) (be sure to measure from the edge of disk to edge of zone of inhibition) 13. Which antiseptic is the most effective against Staph aureus? 14. Which antiseptic is the most effective against Pseudomonas aeruginosa?

Explanation / Answer

1. Iodine is the most effective antiseptic against S.aureus.

2. Phenol is the most effective antiseptic against P.aeruginosa.

The purpose of this lab is to identify the the antiseptic agents that are most effective against the given pathogens. This would help by identifyting the agents that would help in treatment against these organisms. The observation and conclusion can be given by the exact measurements of the zone of inhibitions. These zones of inhibitions give the area where the Pathogen is not allowed to grow or is inhibited by the antiseptic agent.

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