Besides expressing a protein, transfection of a plasmid can also A. increase pla
ID: 84882 • Letter: B
Question
Besides expressing a protein, transfection of a plasmid can also A. increase plasmid concentration in the cell. B. be transferred by conjugation. C. come together at cos sites. D. be packaged into phage heads for further infection. E. none of the above A repressor protein would have which of the following effects on repressible genes with a negative control mechanism? A. stimulate transcription in the presence of a corepressor B. turn off transcription in the presence of an inducer C. stimulate transcription in the presence of a coactivator D. turn off transcription in the presence of a corepressor E. no response In the tryptophan operon, the repressor actively binds to the operator when: A. tryptophan binds to an allosteric site on the repressor. B. tryptophan levels are low. C. lactose levels are low. D. there is a great need for proteins. E. allo-tryptophan levels are low. A repressible operon is usually controlled by: A. an inactive repressor that allows it to be in the "on" state. B. the supply of the precursor product for the enzymes. C. an inactive repressor that keeps it in the "off" state. D. tryptophan. E. being turned "on, " usually by the end product of the pathway.Explanation / Answer
15. Transfection of a plasmid can increase plasmid concentration in the cell as the plasmid will be introduced into the cell by this method.
16. A repressor protein will turn off transcription in the presence of a corepressor. For e.g. tryptophan as corepressor and turns off the tryptophan operon
17. In the tryptophan operon the repressor actively binds to the operator when:
A: tryptophan binds to an allosteric site on the repressor as in the case of tryptophan as this leads to a conformational change in the repressor.
18. A repressible operon is usually controlled by:
E: is being turned on usually by the end product of the pathway as this is a case of feedback inhibition.
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