Which of the following media can be used to determine Hydrogen sulfide productio

Question

Which of the following media can be used to determine Hydrogen sulfide production? a. SIM medium b. Kligler's Iron agar slant c. Russel Double Sugar (RDS) slant d. A and B can both be used satisfactorily e. A and C can both be used satisfactorily Indole is a by-product of the metabolic breakdown amino acid Tryptophan used by some micro-organisms. The presence of Indole of the by using which of the following reagents? a. Barritt's reagent b. Oxidase reagent. c. Ferric Chloride solution d. Kovac's reagent e. Gram's Iodine What test reagent is needed to demonstrate the results of Voges Proskauer test? a. Barritt's reagents A and B b. Methyl red c. Nitrate reagents A and B d. Kovac's reagent e. Ferric chloride solution Fermentation may be defined as the incomplete oxidation of: a. Starch in the presence of oxygen b. Starch in the absence of oxygen c. Glucose or other monosaccharides in the presence of oxygen d. Glucose or other monosaccharides in the absence of oxygen e. Urea in the absence of oxygen A Staphylococcal isolate found to be positive for mannitol fermentation is likely to be a. Staphylococcus aureus b. Stahylococcus epidermidis c. Staphylococcus saprophyticus d. Either A or C e. Either A or B If optochin sensitivity is indicated in a blood agar plate inoculated with Streprococcus, the bacteria is likely a. Streptococcus agalactiae b. Streptococcus pyogenes c. Viridans streptococcus e. Streptococcus pneumoniae e. Streptococcus faecium Shigella and Salmonella are a. Both positive for lactose fermentation b. Both positive for hydrogen sulfide production c. Both negative for urease activity d. Both negative for indole production Mannitol Salt Agar contains mannitol, 7.5% sodium chloride, and phenol red. The role of sodium chloride in the medium is a. To select for all gram positive bacteria b. To select for all gram negative bacteria c. To inhibit organisms other than Staphylococci d. None of the above is applicable

Explanation / Answer

8) Option d) Both the SIM and Klinger Iron slant can be used satifactorily as a medium for the determination of H2S

9) Option D) Kovac's reagent. It can be used to determine the presence of Indole.

10) Option A) Barritt's Reagent A and B.

11) Option D) Glucose and other macromolecule in absence of oxygen.

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