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HELP! BIOCHEMISTRY! Choose the buffer characteristic that fails to meet the crit

ID: 844831 • Letter: H

Question

HELP! BIOCHEMISTRY! Choose the buffer characteristic that fails to meet the criteria. Choose the best answer given the information.

The criteria for acceptable biological and biochemical buffers were established by studies conducted by Norman Good etal. in 1966. These criteria included a pKa within physiological range soluble in water membrane impermeability minimal salt effect minimal temperature and concentration effects on dissociation minimal interactions with cations chemical stability no/minimal, or well-documented, UV light absorbance at certain wavelengths easy to prepare Choose the buffer characteristic that fails to meet the criteria. Choose the best answer given the information.

Explanation / Answer

a) acetate buffer michaelis buffer

ineffective near physiological pH

b) Tris(hydroxymethyl)aminomethane

effect of temperature on dissociation is too high