Please also explain why you picked your answer. When carrying out the analysis o

Question

Please also explain why you picked your answer.

When carrying out the analysis of morphine and its metabolites in postmortem blood, solid phase extraction (SPE) is often used in the sample preparation step prior to analysis. Which ONE of the following describes the mechanism of how this preparation step is used? A. The SPE cartridge allows the morphine and its metabolites to remain in a suitable solvent. B. Morphine and its metabolites are retained on the chromatographic media of the SPE cartridge before being washed out with an appropriate solvent. C. The SPE cartridge acts as a simple filter, removing large particles that would interfere with the HPLC column. D. Morphine and its metabolites are not in the correct ionic form, therefore the SPE cartridge will allow the chemistry of the analytes to be modified for analysis via HPLC. A volatile substance is usually used to dissolve a sample before spotting onto the TLC plate. Which ONE of the following is the reason why a volatile solvent is usually used? A. To enable the sample to be separated in order to evaporate across the TLC plate. B. The TLC plate would usually be in the dry state and requires a solvent to prepare the surface. C. To avoid excess spreading of the sample across the TLC plate. D. The TLC plate requires a volatile solvent that would allow the non volatile sample to be in the correct state for analysis.

Explanation / Answer

16. The Solid phase extraction is often usedin sample preperation step prior to analysis, because it acts as a simple filter, removing large particles that would interfere with the hplc column. SPE uses the affinity of solutes dissolved or suspended in a liquid known as a mobile phase for a solid through which the sample is passed known as stationary phase to seperate a mixture into desired and undesired components.

17. A volatile substance is usually used to dissolve a sample before spotting onto plate, because the plate requires a volatile solvent that would allow the non volatile sample to be in correct state for analysis. The TLC technique is mainly used to seperate non volatile mixtures.

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