You have an impermeable membrane bag (i.e. nothing gets through its walls). You

Question

You have an impermeable membrane bag (i.e. nothing gets through its walls). You insert ACh receptors into the wall of this bag. The ACh receptors are from a synapse that forms IPSPs. Inside this bag there is 3% Na+, 4% K+ and 2% Ca2+. At time=0 sec you place the bag into a beaker of pure water. At time=30 sec, you add acetylcholine to the beaker. At time=60 sec, you add acetylcholinesterase the beaker.

Describe what will occur during your experiment.

Draw graph showing what happens to the concentration of each ion in the bag from 0 sec to 90 sec

Explanation / Answer

The AChR or acetyle choline receptor is a non-selective cation channel, meaning that several different positively charged ions can cross through. It is permeable to Na+ and K+, with some subunit combinations that are also permeable to Ca2+ . The bag is impermeable. No ion can pass through. AS it is plased in pure water. There is no ion there.So up on binding to acetyl choline receptor will open. Ions will be diffused out of the bag. Ions will be distributed equally all through beaker untill in 60th sec when acetylcholine estarase will be added.

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