1) If two samples are spotted on a TLC plate and after developing the TLC plate

Question

1) If two samples are spotted on a TLC plate and after developing the TLC plate the two compounds have the same Rf. What conclusions can you draw about the two compounds?

2) Which is a more polar solvent mixture (mobile phase) for TLC, 1:1 ethyl acetate/hexane or 4:1 ethyl acetate/hexane?

3) The following three compounds are spotted on a TLC plate. The TLC plate is developed using 7:3 hexane/ethyl acetate, what will be the order of elution (i.e., which will move furthest up the TLC plate);

alcohol, an aromatic or a carboxylic acid?

Explanation / Answer

1 ) You can conclude that the two compounds may be same or the two compound may have same polarity in the specific medium that is used.

2 )4:1 ethyl acetate/hexane is a better polar solvent mixture than 1:1 mixture as hexane is non polar and ethyl acetate is polar solvent

3 )Carboxylic acid > alcohol > aromatic

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