Drosophila researchers have collected many strains that carry a single recombina
ID: 82933 • Letter: D
Question
Drosophila researchers have collected many strains that carry a single recombinant P element containing a wild-type white gene (a P [w^+] transgene) inserted into a known genomic location. These strains can be used to map the location of any mutant gene in the fly genome. Investigators performed a testcross to map a recessive mutation rough (ro), which causes rough eyes, relative to a P[w^+] element on chromosome 3. Females heterozygous for the P[w^+] on one chromosome 3 and a ro^- mutation on the other chromosome 3 homolog were crossed to ro^-/ro^- males, and the progeny in the following list were obtained. In both the parents and the progeny, the endogenous white gene is the flies have red eyes only if they contain the P[w^+] transgene. The results are as follows: 145 red, smooth (wild-type) eyes 152 white, rough eyes 2 white, smooth eyes 1 red, rough eyes Is this P [w^+] transgene insertion useful for identifying the smooth gene. A. Yes, because it is closely linked to smooth B. Yes, because it is not linked to smooth C. No, because it is closely linked to smooth D. No, because it is not linked to smooth E. NoneExplanation / Answer
(A). Yes the P[w+] transgene insertion is useful for identifying the smooth gene. The red eyes are expressed only if the progeny has taken up the P[w+] transgenes. There are many progeny produced with red eyes.
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