Let\'s say that I found a novel species of bacteria and would like to see if it
ID: 82815 • Letter: L
Question
Let's say that I found a novel species of bacteria and would like to see if it has the gene for Beta-galactosidase. I design PCR primers to amplify beta-galactosidase, if it is present. The techniques that will be used for this experiment are PCR followed by gel electrophoresis. If he runs a gel and doesn't see any bands for beta-galactosidase that means that either (1) the bacteria doesn't have beta-galactosidase or (2) that something is wrong with his PCR primers or design. To be able to tell which one is going on, he could use a positive control. What would be a good positive control for this experiment.Explanation / Answer
A positive control that can be used for the detection of Beta galactosidase is the use of e.coli bacterial culture.E.coli has been shown to be positive for the presesnce of beta galctosidase. E.coli MG 1655 can be uesd as an positive control as it contains beta galactosisdase gene and will show the presence of beta galactosidase band during electrophoresis.
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