Joshua and Esther Lederberg devised a simple technique, called the replica plati

Question

Joshua and Esther Lederberg devised a simple technique, called the replica plating method, to test colonies of microorganisms under different growth conditions. A master plate, containing the colonies to be tested, is pressed onto a sterile velvet surface, depositing some cells from each colony in the same pattern as on the master plate. Then fresh culture plates are pressed onto the velvet to pick up cells in the same pattern. The transferred cells can be grown on different media or at different temperatures than the original colonies. Suppose the plates below represent a master plate and replicate plates. Which of the colonies (circled numbers) would you conclude have growth mutations? What kinds of mutations do you suspect? Explain your reasoning.

Explanation / Answer

Colony no 3 and 4 , and colony no 6 & 8 have mutations . In colony 3 & 4it may be an amino acid deficient mutation has occur ( depends on which component is dropped out from natural SD media. But colony no 6 & 8 are temperature sensitive mutants which can grow in lower temperatures but can't grow in high temperature.

Related Questions

Navigate

• Browse (All)
• Browse J
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.