apling learning The data below were collected for an enzyme-catalyzed reaction.

Question

apling learning The data below were collected for an enzyme-catalyzed reaction. This graph is provided for your use [S] (mM) Vo (mM- s only. It will not be graded. (It is not necessary to use a graph to answer.) 0.20 0.37 050 Click on the graph to 0.83 activate the Tools 2.00 2.22 menu 0.6 4.00 3.08 Choose "Point," and then click a place on the graph to place Determine max and Km for this reaction. the point. To move a point, choose "Select," and Number then drag the point. Use "Select" to mM. scroll over a point to see its coordinates, or to see the equation of a line. Number Use the option 0.0 -2 -1 0 3 5 "Line" to draw a line. mM

Explanation / Answer

Vmax is the rate of reaction when enough substrate complitly fill ie,satutated enzyme site .here

Vmax = 3.09mM.s-1

Km is substrate concentration which occupied half the active site of enzyme.Vmax /2 =3.09 /2 =1.545mM.s-1

Km = 1.012

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