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Suppose a thin layer of bovine serum albumin (BSA) protein solution was layered

ID: 812162 • Letter: S

Question

Suppose a thin layer of bovine serum albumin (BSA) protein solution was layered onto the surface of the sample (assume no mixing occurs). You decide to come back when the particle reaches the bottom due to sedimention by gravity. If BSA weighs 66,000 g/mole and has a friction coefficient of 6.81 x 10-8 g/s. You have been waiting for 6.216x1011 s.

You return to find that instead of reaching the bottom, your layer of BSA protein is distributed throughout the entire cell. Why? 5. Suppose you had a cell filled with pure water to a height of 1 cm and placed a particle just below the surface of the water. The sample is maintained at 20 degree C

Explanation / Answer

BSA coated particles interact with water, because it is polar. A long waiting time means this BSA coated particle will not sediment or settle at bottom of the cell.

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