Which is NOT a difference between next generation sequencing (NGS) and Sanger se

Question

Which is NOT a difference between next generation sequencing (NGS) and Sanger sequencing? a. Sequence read length b. Sequence reaction chemistry c. Read-out frequency per sequencing reaction d. Difficulty to sequence through repetitive sequences DNA binding proteins often can read the sequence of nucleotides along the DNA without having to separate the chains. How do they do this? a. The DNA binding proteins often contain domains that fit into the DNA grooves. b. The conformations of the deoxyribose sugars reflect the DNA sequence. c. Depending on the base pair, there are different H available for H bonding in the major and minor grooves d. A and C A frameshift mutation could result from a. a base insertion b. a base deletion c. either an insertion or deletion of a base The three tRNA binding sites found on the ribosome (A, P and E) are a. found on the small subunit b. found on the large subunit c. span both the large and small subunit d. none of the above

Explanation / Answer

24) option B. sequence reaction chemistry. there is a difference in reading length, readout frequency per sequencing reaction and difficulties in reading repetitive sequences.

25) option D. A and C. DBP contains the domains called DNA binding domains which fits in either major groove or minor grooves of DNA and binds with different H-bonding.

26) frameshift mutation results by either insertion or deletion of a base. for example

aaa bbb ccc ddd eee fff is a DNA sequence. now insertion of a base Z.

aaa bbb ccc Zdd dee eff f

and deletion of base d from sequence

aaa bbb ccc dde eef ff.

27) option C. span both the large and small subunit. for more info read this article

http://www.sciencedirect.com/science/article/pii/S0092867402006190

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