Many DNA binding proteins H-bond with the major groove on the DNA using an alpha
ID: 81102 • Letter: M
Question
Many DNA binding proteins H-bond with the major groove on the DNA using an alpha helix. This H-bond results in denaturation of the DNA. a. True b. False Suppose there is a mutation in the lac operator preventing the lac repressor from binding. Which of the following situations will result in activated transcription? a. Low glucose, low lactose b. High glucose, low lactose c. High glucose, high lactose d. A and C e. Band C You have introduced a mutation into AraC that causes it to lose its ability to bind arabinose. How will this affect the araBAD operon? a. The operon will always be transcribed b. The operon will never be transcribed c. The operon will be regulated normally d. Glucose will now regulate the operon e. Lactose will now regulate the operon Imagine that you discover a bacterial operon involved in the synthesis of biotin. This operon is regulated by a repressor protein that binds to an operator sequence. When biotin binds the repressor, the DNA binding sequence of the protein is revealed. Predict how biotin will influence repressor activity. a. When biotin binds to the repressor, the repressor cannot bind to the operator. b. when biotin binds to the repressor, the repressor can bind to RNA polymerase. c. When biotin binds to the repressor, the repressor cannot bind to RNA polymerase. d. When biotin binds to the repressor, the repressor binds to the operator.Explanation / Answer
Answer:
1) Many DNA binding proteins H-bond with the major groove on the DNA using an alpha helix. This H-bond results in denaturation of the DNA.
2) Suppose there is a mutation in the lac operator preventing the lac repressor from binding. Which of the following situations will result in activated transcription
a) Low glucose, low lactose - Correct Answer
b) High glucose, low lactose
c) High glucose, high lactose
d) A and C
e) B and C
3) You have introduced a mutation into Aarac that causes it to lose its ability to bind arabinose. How will this affect the araBAD operon?
a) The operon will always be transcribed
b) The operon will never be transcribed - Correct Answer
c) The operon will be regulated normally
d) Glucose will now regulate the operon
e) Lactose will now regulate the operon
4) Imagine that you discover a bacterial operon involved in the synthesis of biotin. This operon is regulated by a repressor protein that binds to an operator sequence. When biotin binds the repressor, the DNA binding sequence of the protein is revealed. Predict how biotin will influence repressor activity.
a) When biotin binds to the repressor, the repressor cannot bind to the operator - Correct Answer
b) When biotin binds to repressor, the repressor can bind to RNA polymerase
c) When biotin binds to the repressor, the repressor cannot bind to RNA polymerase
d) When biotin binds to the repressor, the repressor binds to the operator.
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