In order to make a Beer\'s law plot for the blue basic form of bromothymol blue

Question

In order to make a Beer's law plot for the blue basic form of bromothymol blue (BB?) we will need to make four solutions in the concentration range 4 10?5 M and 4 10?6 M. Indicate how much stock solution (4.00 10?4 M) should be used to get the desired concentration. (answer need to have 3 decimal places)

To make 10.00 mL of 5.00 10?6 M BB? we would transfer  mL to of stock solution volumetric flask and dilute to the mark with 0.1M NaOH.

To make 10.00 mL of 7.00 10?6 M BB? we would transfer  mL of stock solution to volumetric flask and dilute to the mark with 0.1M NaOH.

To make 10.00 mL of 1.0 10?5 M BB? we would transfer  mL of stock solution to volumetric flask and dilute to the mark with 0.1M NaOH.

To make 10.00 mL of 3.0 10?5 M BB? we would transfer  mL of stock solution to volumetric flask and dilute to the mark with 0.1M NaOH.

Explanation / Answer

Given molarity of stock solution = 4E-4M

1. To make 10.00 mL of 5.00E-6 M BB? we would transfer  mL to of stock solution volumetric flask and dilute to the mark with 0.1M NaOH.

answer: use M1V1 = M2V2

10mL x 5E-6M = V2 x 4E-4M

V2 = 0.125mL

So, volume needed = 0.125mL

2. To make 10.00 mL of 7.00E-6 M BB? we would transfer  mL to of stock solution volumetric flask and dilute to the mark with 0.1M NaOH.

answer: use M1V1 = M2V2

10mL x 7E-6M = V2 x 4E-4M

V2 = 0.175mL

So, volume needed = 0.175mL

3. To make 10.00 mL of 1.00E-5 M BB? we would transfer  mL to of stock solution volumetric flask and dilute to the mark with 0.1M NaOH.

answer: use M1V1 = M2V2

10mL x 1E-5M = V2 x 4E-4M

V2 = 0.25mL

So, volume needed = 0.25mL

4. To make 10.00 mL of 3.00E-5 M BB? we would transfer  mL to of stock solution volumetric flask and dilute to the mark with 0.1M NaOH.

answer: use M1V1 = M2V2

10mL x 3E-5M = V2 x 4E-4M

V2 = 0.75mL

So, volume needed = 0.75mL

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