A. You must isolate mutant cells defective in the DNA-damage checkpoint, but you

Question

A. You must isolate mutant cells defective in the DNA-damage checkpoint, but you find that under normal laboratory conditions, the mutant cells grow and divide at the same rate as wild-type cells. Why doesn’t the defect in the DNA-damage checkpoint have a large effect on cell growth and division rates under normal conditions?

B. Please describe an experimental condition in which wild-type cells and mutant cells defective in the DNA-damage checkpoint would show a difference in cell growth and division rates.

C. How would this condition affect the cell growth and division rates in the wild-type and mutant strains from Part A?

Explanation / Answer

A. A cell cycle checkpoint is defined as the stage in which cell decides whether to proceed further for cell division or not. DNA damage check point does not have large effect on cell growth and division rates because cells enter the mitosis before repairing the DNA. But the cells will die after cell division.

B. If the cell is deficient of dNTPS, then the large effect on cell growth and division rates can be observed.

C. Absence of dNTPs will not allow DNA replication as well as prevent the repair of the damaged DNA.

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