1. Shown below is the absorbance spectrum of CBBG in the presence of a large exc
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Question
1. Shown below is the absorbance spectrum of CBBG in the presence of a large excess of BSA. The x axis shows wavelength (in nm) and the y-axis is absorbance - the y-axis is arbitrary since the absorbance depends on the concentration. You'l1 notice that the maximum of this absorbance spectrum occurs at 595 nm, which is where you measured the absorbance of the complex. 0.5 0.0 400500600700800 In theory, you should observe a sharp, narrow peak. Explain. However, experimentally, you observe a broad peak. Explain why this is the case. (8 points) 2. CBBG dye is a red-brown color when unbound to proteins. The protein-dye complex is blue. Describe how the color change relates to the relative energies of the two forms. (6 points)Explanation / Answer
1.
From quantum mechanics the intensity of an electron transition is proportional to the oscillator strength or transition dipole moment (see what Wikipedia has to say about these terms): ?fi = ??f??id? where ?f and ?i are the final and initial wavefuntions and ? is the electric dipole operator.there is a large parity accompanying the ? ? ?* transition (large dipole moment change) so that it is strongly allowed.
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