You are interested in examining the Psf gene. Psf is normally produced known tha

Question

You are interested in examining the Psf gene. Psf is normally produced known that when cells are exposed to high levels of both calcium (Ca^2+) and magnesium (Mg^2+) MetA, MetB, and MetC are important for binding to the promoter of the psf gene and are involved in regulating its transcription. MetA binds to the "A" site in the promoter region MetB to the "B" site, and MetC to the "C" site. You create binding-site mutations the A, B, and C sites and observe what happens to transcription of the Psf gene. Your results are summarized in Table Q6. Which of the proteins are likely to act as gene activators? Which of the proteins are likely to act as gene repressors? Which transcription factors are normally bound to the Psf promoter in the presence of Mg^2+ only? Which transcription factors are normally bound to the Psf promoter in the presence of both Mg^2+ and Ca^2+?

Explanation / Answer

1. Both A and C act has a activator of psf gene.

2. Only B present but no expression happen in the psf gene. So it is a repressor.

3. Only A. At mg2+ only A will be activate the psf gene.

4. A and C bind to psf promoter in presence of both Mg2+ and Ca2+.

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