If I were to say, \" Protein X can cause cellular transformation when expressed

Question

If I were to say, "Protein X can cause cellular transformation when expressed in a normal lung cell line," what experimental approach you would use to prove this hypothesis? Include:

a) the experimental approach.

b) a general outline of the procedure for that approach, i.e. an overview of how the protocol is conducted, including critical reagents or steps

c) expected results, including any specific marker proteins associated with the assay that would change or vary.

Nothing too lengthy, please and thank you!

Explanation / Answer

This can be find out by the help of any of the method

By using reporter gene

By recombinant DNA method I.e. nucleic acid hybridisation

In reporter gene method they change the colour from blue to green when a specific protein is reported this reporter gene is called green fluorescent protein. The light is emited by the enzymatic activity of luciferase upon the luciferin component.

In nucleic acid hybridisation to detect a specific sequence its complementary sequence is inserted, if desired sequence will be present it will make d.s. sequence with the provided sequence.

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