1.A segment of DNA has a strand containing the sequence of 5\' GCTATGT 3\'. What

Question

1.A segment of DNA has a strand containing the sequence of 5' GCTATGT 3'. What is the sequence of the complementary strand? Be sure to indicate polarity of the strand sequence. If the strand given is above is sequenced using the Sanger (di-deoxy) method, sketch/list the pattern of fragments generated in the sequencing process. Be specific.

2. For a coding DNA sequence 5' TCCTGCGTACTG 3', what will be the template DNA sequence, the messenger RNA transcribed and for what peptide sequence will it code?

3. A peptide is known to have a sequence of LACFM. What is a possible mRNA sequence for this peptide? Is it the only possible sequence? Explain? For an mRNA sequence that codes for this peptide, what will be the coding DNA that corresponds to it? What will be the template DNA? Again, give base sequence(s) and indicate clearly all strand polarities.

4. A restriction nuclease recognizes the sequence

5' ATGGATCG 3'

3' GCTAGGTA 5'

Locate the axis of symmetry. What are the sticky ends produced if the enzyme cleaves between GG? Be specific.

Explanation / Answer

1.

3'CGATACA5'

2.

3'AGGACGCATGAC5 --template DNA;3'AGGACGCAUGAC5--mRNA

3.

5'CUUGCAUGCUUUAUG3'

THERE ARE MANY possible sequences since an aminoacid can be coded by one or more codons 5'CTTGCATGCTTTATG3'----TEMPLATE dna 3'GAACGTACGAAATAC5'---BASE SEQUENCE

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