I am having trouble answering the following questions for my lab hw from my Prin

Question

I am having trouble answering the following questions for my lab hw from my Principles of physiology class. The following is the questions. I have also posted pictures of the lab and collected data.

1. Write a short paragraph for each step of the experimental approach that shows that you fully understood the hypothesis you were testing; that is to say, you should know how and why you did everything you did (combine 1. and 2. for each section)

.

BIO 361: Active Transport Lab Learning objectives: After completing the lab exercise and analyzing data at home, students will be able to... 1. Describe the passive solute and water fluxes encountered by a freshwater vertebrate 2. Explain cellular mechanisms by which a freshwater amphibian absorbs solutes from the environment 3. Describe the difference between a hypothesis and a prediction and give a physiological example of each Introduction: Active transport is a fundamental process occurring i many tissues, cells, and organelles, which allows for movement of materials against an electrochemical gradient In this experiment, we will examine the role of active transport in osmoregulation using the abdominal skin of a frog. Fresh water frogs live in an environment that is hypoosmotic to their body fluids, so they are faced with the problems of passive salt loss and passive water gain. The frog is able to excrete excess water by producing dilute urine in the kidneys. The skin also plays a role in osmoregulation by actively transporting salts back into the blood When a charged ion is transported in one direction across a membrane (or epithelium), an electrochemical potential difference is established. This potential difference, or voltage, can be measured by placing an electrode in the solution on one side of the membrane (or epithelium) and another electrode on the other side of the membrane, and connecting both electrodes to an oscilloscope or other device that measures voltage. If a voltage is measured, it indicates that transport is occurring and unequal numbers of positive and negative ions are crossing the membrane. By applying solutions containing different salts to one or the other side ofthe membrane, it may be possible to deduce which ions are being actively transported by those cells

Explanation / Answer

In rder to meausre active transport across the bull frog epithelium, very fist we scrap bull frog skin which we will keep in Ringer's solution which is a kind of isotonic solution that maintained viability and function of tissue. in order to provide the living enviornment to the skin even ater it is removed from the bullfrog we placed the skin in chamber containing ringer's solution.

We then take the using chamber which is divided into different compartments in order to test the active transport across the epithelium of bull frog by placing different concentration of solution in different chamber that help to understand the ion specificity on the influence of change in concentration across the membrane dependent on metabolic energy measured by oscilloscope.

Change in electrode potential which is indicative of change in concetration based on ion specificity movement. Thus skin is bathed with Ringer's solution on one side and 0.1 M KCl solution on other side. Thus maintaiing ion concentration gradient across the membrane. Since we are placing 0.1 M KCl solution in chamber 1 and since KCl carry chloride ion having negative charge thus electrode from chamber 1 is attached to negative terminal of the oscilloscope and celectrode from hole 6 is attached to + terminal of the oscilloscope.

With the help of oscilloscope difference in the electrochemical gradient across the two side of the chamber is determined, dipicting electrochemical potential difference across the two side of the membrane.

Point to note that to maintained osmolarity across the membrane, the solution has to keep on changing on either side of the skin. while changing solution it is important to thoroughly rinse any residue of that ion off of the skin.

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.