hexapeptide contains equimolar amounts of met, cys, phe, glu, ser and arg Cleava

Question

hexapeptide contains equimolar amounts of met, cys, phe, glu, ser and arg

Cleavage of the hexapeptide with trypsin yielded two products: a pentapeptide and free cys

Cleavage of the hexapeptide with chymotrypsin yielded two product peptides. One was a dipeptide containing cys.

Cleavage of the hexapeptide with CNBr yielded two product peptides. One of these was a dipeptide containing glu

Based on above information, what is the amino acid sequence of the hexapeptide? (Be careful: no partial credit on this problem.______ ______ ______ ______ ______ ______

(explain)

Explanation / Answer

Trypsin cleves after Lys or Arg residue , hence peptide has Arg at 5th position and Cys at last

Chymotrypsin cleves after Tpr, Tyr , Phe hence Phe present at 4th psoition , since afetr clevage we left with two parts where 1 part is dipeptide containing Cys i.e Arg-Cys

CnBr cleves after Met group , given clevage by CnBr gave a dipeptide containg Glu hence 1residue is Glu and second is Met

total sequence is Glu-Met-ser- Phe-Arg-Cys

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