1. You are performing Sanger sequencing on a DNA molecule. You prepare the react

Question

1. You are performing Sanger sequencing on a DNA molecule. You prepare the reaction mixture correctly except that you only add on dideoxynucleotide triphosphate, ddTTP (you did not add ddATP, ddCTP, and ddGTP). The DNA to be sequenced and primer are below: Template 5’-GCACCTCCCAAATCTATGGCCCA-3’ 3’-CGTGGCGGGTTTAGATACCGGGT-5’ Primer: 5’-GCACCTCCC-3’ Based on the template sequence and primer, how many bands to you expect to see when the sequencing reaction is run on a gel? State the length (in bp) of each of the DNA fragments. I need the number of the bands with their length in bp

Explanation / Answer

3’-CGTGGCGGGTTTAGATACCGGGT-5’ primer will bind on only this template

Product will terminate when A will come in extension sequence means when T will come after primer binding in template

3’-CGTGGCGGGTTTAGATACCGGGT-5’

products.......

1.5’- GCACCTCCCA 10bp

2.5’-GCACCTCCCAA 11bp

3.5’-GCACCTCCCAAA 12bp

4.5’-GCACCTCCCAAATCTA 16bp

5.5’-GCACCTCCCAAATCTATGGCCCA 23bp

Total 5 band will be observed which are 10, 11, 12, 16 and 23bp

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