1. What kind of plates would you prepare to determine if an E.coli strain is aux
ID: 78621 • Letter: 1
Question
1. What kind of plates would you prepare to determine if an E.coli strain is auxotrophic for histidine
2. Suppose that you want to insert into a strain of photosynthetic bacterium Rhodobacter sphaeroides a gene that encodes a protein that confers resistance to chloramphenicol. Rhodobacter sphaeroides cannot be made competent, so they cannot take up the gene directly from the environment. However, you have a plasmi (F+ plasmid) that can be maintained by these bacteria. What natural process would you use to genetically alter Rhodobacter sphaeroides to be chloramphenicol resistant?
3. Hydrogen bonds between complementary nucleotides maintain the structure of double stranded DNA, since they hold the two strands together. Why couldn't the two strands be effectively linked by covalent bonds?
Explanation / Answer
Answer:
1. Minimal medium + or - histidine can be used to determine if an E.coli strain is auxotrophic for histidine.
Wild-type cells will grow with or without histidine. Histidine auxotrophs will not be able to grow without histidine in this screen.
(Since there are more than 1 question, the first question have been answered according to the rules of Chegg)
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