K av for trypsin = 0.13 mL K av for benzamidine = 0.52 mL specific activity, uni
ID: 78331 • Letter: K
Question
Kav for trypsin = 0.13 mL
Kav for benzamidine = 0.52 mL
specific activity, units/ mg uninhibited trypsin 5.84* 10-2 benzamidine-inhibited trypsin 5.86* 10-3 purified trypsin 4.59* 10-2 4. If the separation of benzamidine from trypsin was not completely successful, one should, in principal, be able to calculate the amount of inhibitor remaining in the trypsin fraction from the activities of the column fraction and the uninhibited trypsin. What additional information would you need in order to do this calculation?Explanation / Answer
following information is required :
1. observed specific activity of trypsin in the fractions
2. observed specific activity of benzamidine in the fractions
3. total volume of the fractions
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