If you wanted to isolate a copy of the gene that codes for a protein found in th

Question

If you wanted to isolate a copy of the gene that codes for a protein found in the stomach, could that gene be located in cheek cells? Explain your reasoning. Once the membranes have been dissolved, the DNA is released into the solution, but so are many other types of cellular molecules. List some types of molecules besides DNA that you would expect to find in a cell. How could you test whether you were actually collecting cells from your cheeks? What piece of laboratory equipment might you use? Did you think your DNA would be visible after you had broken open your cells? Why or why not? Where do you think you would find proteases in your body? In which cellular compartment do you expect to find your genomic DNA? Match the outcomes on the left with the laboratory steps on the right. _________ Harvest the cells A. Gently chew the insides of your mouth and rinse vigorously with water __________ Dissolve cell membranes B. Add protease, incubate at 50 Degree C __________ Precipitate the DNA C. Mix in a detergent solution __________ Break down proteins D. Layer cold alcohol over cell extract

Explanation / Answer

Ans 1) Yes, if you are trying to find a gene for a protein found in the stomach, it is possible to find the gene in cheek cell but most probably it will not be functional. The reason why the gene in stomach and check cell will be same or present is because all the cells in the body have the same genetic composition. It means although it is present in cheek cells but it will not be functional.

Ans 2) Apart from the DNA, the other cellular molecules that will be released in the solution once the membrane is disrupted are carbohydrates, RNA, proteins, lipids etc.

Ans 3) The cheek cells can be extracted or swapped out using a toothpick and in order to observe them, regular bright field microscope is fine. The transmission electron microscope or scanning electron microscope is better to check the details.

Ans 4) Simply by breaking open the cell, DNA is not visible since it is localised in the nucleus of the cell. It requires extraction using various steps to be visible under microscope.

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