Which of the following statements regarding thin layer chromatography are TRUE?

Question

Which of the following statements regarding thin layer chromatography are TRUE? - Partition chromatography separates compounds based on how they distribute themselves between the stationary and mobile phases. -The Rf value will change if the adsorbent material is changed. -In thin layer chromatography, a polar organic compound will be repelled by the adsorbent and therefore travel further up the plate than a non-polar compound. -Covering the sample jar is done to prevent fingerprints on the slide. -In thin layer chromatography, a polar organic compound will be attracted by the adsorbent and therefore travel not as far up the plate as a non-polar compound -Sample spots should be approximately 1 cm in diameter.

Explanation / Answer

The Rf value will change if the adsorbent material is changed.

Sample spots should be approximately 1 cm in diameter.

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