You have isolated a bioactive hexapeptide from a newly discovered locust, Locust

Question

You have isolated a bioactive hexapeptide from a newly discovered locust, Locusta bullus. You have carried out a series of experiments to determine the sequence of this peptide. Experiment 1: The peptide does not react with the Edman reagent. Experiment 2: Complete acid hydrolysis yielded Val, Glu, Asp, Lys, acetic acid, and ammonia. The molar ratios of the products were 2 Val, 2 Asp, 1 Glu, 1 Lys, 1 NH3, and 1 CH3COOH. Experiment 3: Digestion of peptide with trypsin yields free Asn and pentapeptide. The digestion went to completion and trypsin cleaves C-side of Lys and Arg. Experiment 4: Digestion with Asp-N protease yields N-acetylglutamic acid and pentapeptide. The digestion went to completion and Asp-N protease cleaves on the N-side of Asp. The sequence of the peptide is: A. N-Acetylglutamic acid-Val-Val-Asp-Lys-Asn B. Val-Val-Lys-N-Acetylglutamic acid-Asp-Asn C. Val-Lys-Val-N-Acetylglutamic acid-Asn-Asp D. N-Acetylglutamic acid-Asp-Val-Val-Lys-Asn E. N-Acetylglutamic acid-Lys-Asn-Asp-Val-Val

Explanation / Answer

E. N-Acetylglutamic acid-Lys-Asn-Asp-Val-Val

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