Suppose you are mapping the distance between the locus A and the centromere in N

Question

Suppose you are mapping the distance between the locus A and the centromere in Neurospora. You obtain nine types of asci presented below. Some of these asci indicate that ‘gene conversion’ phenomenon associated with the strand exchange without a mismatch repair may have taken place.

A. Which types of asci indicate this? (see digram below..)

1 2 3 4 5 6 7 8 9
a a a a a A A A a
a a a a a A A A a
A a a A a a A A A
A A A A a a a a A
a A a A A a a A A
a A A A A a a a A
A A A A A A a a a
A A A A A A a a a


The answer is 3,8... please show how to acquire this number.

B. With regards to the previous question, which types of asci are compatible with ‘gene conversion’ that involves a mismatch repair in one or two heteroduplex DNA molecules?
The answer is 2, 4, 7..please show how to acquire this number.

Explanation / Answer

Gene conversion is a nonreciprocal transfer of genetic information. DNA sequence information is transferred from one DNA helix (which remains unchanged) to another DNA helix, with sequence is altered.

When mismatch does not take place, there will be change in the sequence of the DNA.

Among the given ascii, two of them have disturbed sequence. They include-

3 -a a a A a A A A - it can be repaired to produce a a a A A A A A

8 -A A A a A a a a - it can be repaired to produce A A A A A a a a

b). The ascii which undergone the mismatch repair are

2 -a a a A A A A A - without repair the strand will be a a a A a A A A

4 -a a A A A A A A - without repair the strand will be a a A a A A A A

7 -A A A a a a a a - without repair the strand will be A A A a A a a a

The remaining are the parental types with gene conversion.

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