The DNA content of an individual cell can be measured by applying a DNA-specific

Question

The DNA content of an individual cell can be measured by applying a DNA-specific dye to the cell and then passing it through an instrument that measures the staining intensity. A new drug was tested on a population of rapidly dividing tumor cells, and the DNA contents of the treated cells were analyzed and compared with those of untreated cells.

2. Explain the data for the untreated cells. Which cells are in G1? What do the data indicate about how much time cells spend in G1 relative to other phases?

I really only need to be able to figure out how to tell which cells are in G1. I've looked all over the internet and haven't found anything even remotely useful.

Dye Intensity % untreated cells % treated cells <10 0 20 10 10 5 20 55 60 30 5 5 40 30 10

Explanation / Answer

Answer: Here is the exlanation and reasons about the G1 phase and other phases of cell cycle.

1. 55% of untreated cells observed with dye intensity 20. Because as per cell cycle phase; G1 phase is the longest phase which was observed in these cells.

2. 5% unterated cells observed with dye intensity 30. Because as per cell cycle phase; G1 phase is the smallest phase which was observed in these cells.

3. 5% unterated cells observed with dye intensity <10. Because as per cell cycle Phase; DNA in these cells are not initiated cell cycle pahses. Thatsswhy dividing tumor cells with induced drug+dye absorption has not done.

As per Beer-Lamberts law of drug intensity analysis="Absorbance" is directly proportional to "concentration" of drug.

Here are the reasons:

The lengths of G1 and G2 differ in cells depending on the individual cell's level of preparedness for proceeding in the cell cycle. Remember, cells can enter G0 for extensive amounts of time during G1 before continuing on to S phase.

If a cell has rapidly undergone adequate cell growth or DNA replication, the time spent in G1 and G2 will be decreased.

G1 is typically the longest phase of the cell cycle. This can be explained by the reality that G1 follows cell division in mitosis; G1 represents the first chance for new cells have to grow.

Cells typically remain in G1 for about 10 hours of the 24 total hours of the cell cycle. The length of S phase varies as per to the total DNA that the meticulous cell contains; the rate of synthesis of DNA is fairly constant between cells and species.

Typically, cells can take between 5 and 6 hours to complete S phase. G2 is shorter, durable only 3 to 4 hours in most cells.

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