You are given a 1:100 dilution of a suspension of a recently isolated bacterioph

Question

You are given a 1:100 dilution of a suspension of a recently isolated bacteriophage able to lyse E. coli strain B. You then make four, successive 1/10 dilutions of this dilution. From the last dilution you make, 0.1 ml is added to a tube containing 5.0 ml of melted top agar. One-tenth ml of a young culture of host organism is also added to the same tube of top agar. After mixing, the tube of top agar is poured onto a plate of green agar. After incubation, 175 plaques are counted. Determine the number of plaque forming units (PFUs) per ml of the original, undiluted bacteriophage suspension.

Explanation / Answer

Ans: 165 plaque forming units (PFUs) per ml of the original, undiluted bacteriophage suspension.

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