Perform a web search to find the tests used in the Enterotube II™ and API-20e™ t

Question

Perform a web search to find the tests used in the Enterotube II™ and API-20e™ test systems for identifying enteric organisms. The lists can be found online by searching the terms “Enterotube II tests list” and “API 20e tests list” in the Google search engine. Use that information to fill in the table below. Be sure all tests from both systems are included in your table (the API20-e contains 20 biochemical tests and the Enterotube II contains 15). Then, check which are included in the VirtualUnknown™ Microbiology software.

The table will need to be expanded to accommodate all the tests for both systems.

Test

Included in

Enterotube II

(check if yes)

Included in

API-20e

(check if yes)

Also included

In this software

(check if yes)

Results for

E. coli in

Software (+/-)

Test

Included in

Enterotube II

(check if yes)

Included in

API-20e

(check if yes)

Also included

In this software

(check if yes)

Results for

E. coli in

Software (+/-)

Explanation / Answer

  

Included in

Enterotube II

(check if yes

Included in

API-20e(check if yes)

Also included

In this software

(check if yes)

Results for

E. coli in

Software (+/-)

Test

Included in

Enterotube II

(check if yes

Included in

API-20e(check if yes)

Also included

In this software

(check if yes)

Results for

E. coli in

Software (+/-)

Glucose Yes Yes Yes + (yelow) Gas Yes No Yes + Lysine(Lysine decarboxylation by lysine decarboxylase) Yes Yes Yes + (purple) Ornithine Yes Yes Yes + (purple) H2S Yes Yes Yes - (colorless) Indole Yes Yes Yes + (red) Adonitol Yes No Yes - ( red) Lactose Yes No Yes + (yellow) Arabinose Yes Yes Yes + (yellow) Sorbitol (fermentation) Yes Yes Yes + (yellow) Voges Proskauer (VP) test Yes Yes Yes -(red) Dulcitol Yes No Yes - (green) Urease Yes Yes Yes - (yellow) Citrate Yes Yes Yes - (green) Phenylalanine Yes No Yes - (green) ONPG (o-nitrophenyl-b-D- galactopyranoside hydrolysis for presence of beta galactosidase enzyme.) No Yes Yes + (yellow) Arginine( Arginine decarboxylation by dihydrolase ) No Yes Yes - (yellow) Tryptophan (Tryptophan deaminase No Yes Yes - (yellow) Gelatinase (production of gelatinase that liquify gelatin) No Yes Yes - (black precipitate) Mannose (fermentation) No Yes Yes - (yellow) Inositol (fermentation) No Yes Yes - ( yellow) Sucrose ( fermentation) No Yes Yes Variable Rhamnose (fermentation) No Yes Yes + (yellow) Melibiose (Fermentation) No Yes Yes + (yellow) Amygdalin (fermentation) No Yes Yes - (red)

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