You are interested in studying transcription factor X (TFX). You test putative f

Question

You are interested in studying transcription factor X (TFX). You test putative functional domains of TFX using deletion mutants in the reporter assay outlined below and using a gel shift DNA binding assay. Regulatory region X (RRX) is the specific cis element that is recognized by TFX. Results of the binding and reporter assays using the various deletion constructs are shown. What conclusion can you make from this data? TFX recruits histone acetyltransferase to activate transcription. Amino acids 1-50 form the activation domain. Amino acids 75-684 are dispensable for activation function. TFX binds both to RRX and to the TATA box. TFX remodels chromatin to allow transcription.

Explanation / Answer

The transcription factor X (TFX) recognizes the specific cis element the regulatory region X (RRX). The reporter gene is constructed with RRX, TATA box and Lac Z gene. When the mutants and wild type are compared, it can be concluded that the TFX binds to both RRX and TATA box so that the Lac Z gene is transcribed. This because, the N terminal mutant lacking the 1 to 49 amino acid sequence does not exhibited the transcription. This indicates the presence of RRX and TATA box in the upstream region to 50 to 823 sequences. Since, the TFX could not bind to RRX and TATA box; the mutant could not express the LacZ gene. Therefore, the option d is correct.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.