Consider a batch culture of bacteria with empirical biomass formula: CH 1.9 O 0.

Question

Consider a batch culture of bacteria with empirical biomass formula: CH1.9O0.6N0.15 The bioreactor is a closed vessel maintained at 37 degree C, into which liquid medium containing 50 mmol glucose (C6H12O6) and an excess of ammonium sulfate was added. The pressurized headspace contained dehumidified air initially. After some time, during which a net growth 1.0 g dry cell weight was achieved, the headspace gas was analyzed. After accounting also for the concentration of dissolved O2 in the medium, it was determined that 60 mmol of total O2 had been consumed. The organism secretes no appreciable amounts of products under these conditions. a. Estimate the yield coefficient of substrate, Y x

Explanation / Answer

Growth yield is the incremental increase in biomass which arises from the utilization of the excess amount of substrate.

Maximum specific growth rate, mm =Y.k

where, Y is the maximum yield coefficient. It is defined as the ratio of maximum mass of cells formed to the mass of substrate utilized.

Yx = yield coefficient of sbstrate

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