HELP PLASE 3500 POINTS Which is the correct order for making cDNA for cloning? Y
ID: 73091 • Letter: H
Question
HELP PLASE 3500 POINTS
Which is the correct order for making cDNA for cloning? You wish to sequence the promoter region of the mouse rab. To do this, you need to construct a library and then search for the gene sequence. You have a DNA probe based on the sequence of the protein coding region. Which of the following strategies would you follow in order to carry this out? If one were to examine the clones in a cDNA library derived from heart muscle cells and the clones in a cDNA library derived from pancreatic cells, you want find that: Which of the following is not an assumption made by the Hardy-Weinberg law? In a particular population at Hardy-Weinberg equilibrium, the frequency of recessive allele b for a particular gene is 0.01. Assuming that there are only two alleles for the gene, what would be the expected frequency for dominant allele B in the next generation? A population consisting of 1876 AA individuals, 1793 Aa individuals, and 5640 aa individuals is established on a remote island. The allele frequencies in this population are:Explanation / Answer
63.
Complementary DNA or cDNA is synthesized by RNA - dependent DNA polymerase or reverse transcriptase isolated from retrovirus. The cDNA synthesis occurs in the sequence of isolation of mRNA, oligo (dT) primer and reverse transcriptase. Option d is correct.
64.
To sequence mouse rab gene promoter, a cDNA library is to be constructed since, DNA probe based sequence of protein coding region is available and promoter is screened by colony hybridization. Hence, option d is correct.
65.
The cDNA library derived from heart muscle cells and cDNA library derived from pancreatic cells have some common cloned cells and also have unique sequences confined specific library but not found in other. Hence, option c is correct.
66.
Hardy - Weinberg law follows the assumptions that populations undergo random mating, no mutation, no migration and no gene flow due to genetic drift or shift. Hence, option b is correct.
67.
The frequency of allele b = 0.01
The frequency of allele B = 1 - 0.01 = 0.99. Hence, option d is correct.
68.
Total number of population = 1876 + 1793 + 5640 = 9309
Frequency of AA (p) = 1876/9309 = 0.2
Frequency of Aa (pq) = 1793/ 9309 = 0.192
Frequency of aa (q) = 5640/9309 = 0.60
But if p = 0.2 and then q = 1 - p = 1 - 0.2 = 0.8. Hence, option b is correct.
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